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LABORATORY TEST DIRECTORY

HRAS Mutation Detection by Pyrosequencing

CPT Code(s): 88381; 81403
Specimen Required: Patient Preparation:
Collect:Tumor tissue.
Specimen Preparation:Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or 5 unstained 5 micron slides. (Min: 3 slides).A Fine Needle Aspirate (FNA) smear may also be submitted. Prepare FNA smear with Diff-Quik or equivalent stain by standard methods (air-dried slides are preferred). Number of slides needed is dependent on the tumor cellularity of the smear. (Min: 1 slide). Slide(s) will be destroyed during testing process and will not be returned to client.Transport block and/or slide(s) in a tissue transport kit (RDL supply # 47808) available online through eSupply using RDL Connect or contact RDL Client Services at (888) 431-7474.A Fine Needle Aspirate (FNA) specimen stored in saline, PreservCyt or CytoLyt may also be submitted.
Storage/Transport Temperature:Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.
Stability:FFPE and FNA smears: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable FNA cells unfixed: Ambient: 2 days; Refrigerated: 2 days; Frozen: Unacceptable FNA cells fixed: Ambient: 3 days; Refrigerated: 14 days; Frozen: Unacceptable
New York DOH Approval Status: This test is New York DOH approved.
Aliases: